Journal
BIOELECTROCHEMISTRY
Volume 101, Issue -, Pages 153-158Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2014.10.003
Keywords
Electrochemical impedance immunosensors; Clostridium difficile toxin detection; Gold nanoparticles; Signal amplification
Funding
- National Science Foundation [CMMI-1030289]
- National Institutes of Health [R01A1088748, R01DK084509, U19A1109776, R56A199458]
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1030289] Funding Source: National Science Foundation
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This work presents a sandwich-type electrochemical impedance immunosensor for detecting Clostridium difficile toxin A (TcdA) and toxin B (TcdB). Single domain antibody conjugated gold nanoparticles were applied to amplify the detection signal. Gold nanoparticles (Au NPs) were characterized by transmission electron microscopy and UV-vis spectra. The electron transfer resistance (Ret) of the working electrode surface was used as a parameter in the measurement of the biosensor. With the increase of the concentration of toxins from 1 pg/mL to 100 pg/mL, a linear relationship was observed between the relative electron transfer resistance and toxin concentration. In addition, the detection signal was enhanced due to the amplification effect. The limit of detection for TcdA and TcdB was found to be 0.61 pg/mL and 0.60 pg/mL respectively at a signal-to-noise ratio of 3 (S/N = 3). This method is simple, fast and ultrasensitive, thus possesses a great potential for clinical applications in the future. (C) 2014 Elsevier B.V. All rights reserved.
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