Journal
BIOELECTROCHEMISTRY
Volume 103, Issue -, Pages 28-33Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.bioelechem.2014.08.009
Keywords
Nanosecond pulsed electric field; Time domain dielectric spectroscopy; Whole blood; Electroporation
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The dielectric spectra of fresh pig whole blood in the beta-dispersion range after exposure to 300-nanosecond pulsed electric fields (nsPEFs) with amplitude higher than the supra-electroporation threshold for erythrocytes were recorded by time domain reflectomety dielectric spectroscopy. The implications of the dielectric parameters on the dynamics of post-pulse pore development were discussed in light of the Cole-Cole relaxation model. The temporal development of the Cole-Cole parameters indicates that nsPEFs induced significant poration and swelling of erythrocytes within the first 5 min. The results also show that the majority of erythrocytes could not fully recover from supra-electroporation up to 30 min. The findings of this study suggest that time domain dielectric spectroscopy is a promising label-free and real-time physiological measuring technique for nsPEF-blood related biomedical applications, capable of following the conformational and morphological changes of cells. (C) 2014 Elsevier B.V. All rights reserved.
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