Journal
BIOCONTROL SCIENCE AND TECHNOLOGY
Volume 25, Issue 5, Pages 583-592Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/09583157.2014.998990
Keywords
aflatoxin; Aspergillus flavus; atoxigenic strain; aflatoxin gene cluster; biocontrol
Categories
Funding
- National Basic Research Program (973 Program) of China [2013CB127802]
- Special Fund for Agro-scientific Research in the Public Interest [201203037]
- National Science Foundation of China [31301478]
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Aflatoxins are toxic and carcinogenic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus. Strains of A. flavus that are non-aflatoxigenic (i.e., incapable of secreting aflatoxins) have proven effective in controlling contamination by these aflatoxin producing species in the field. In the present study, a non-aflatoxigenic A. flavus strain, GD-3, was isolated from a peanut field in Guangdong Province, China. Polymerase chain reaction (PCR) analysis showed that 12 aflatoxin biosynthesis genes (aflT, pksA, nor-1, fas-2, fas-1, aflR, aflJ, adhA, estA, norA, ver-1 and verA) were deleted in GD-3. Co-inoculation with a toxigenic strain, GD-15, at the ratio of 1: 10, 1: 1 or 10: 1 (GD-3: GD-15), showed that GD-3 was capable of reducing detectable aflatoxin levels on three different substrates. This reduction ranged from 33% to 99% and correlated with competitor ratio. These results demonstrated that GD-3 was successful at reducing aflatoxin contamination and showed promise as a potential agent of biocontrol for local farmers.
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