Journal
BIOCONTROL SCIENCE
Volume 20, Issue 2, Pages 125-133Publisher
SOC ANTIBACTERIAL & ANTIFUNGAL AGENTS, JAPAN
DOI: 10.4265/bio.20.125
Keywords
Lipase; Bacillus coagulans; Thermophilic; Central composite design; One-factor-at-a-time
Categories
Ask authors/readers for more resources
The overproduction of enzymes was performed by manipulating the medium components. In our study, solvent-tolerant thermophilic lipase-producing Bacillus coagulans was isolated from soil samples and a stepwise optimization strategy was employed to increase the lipase production using coconut oil cake basal medium. In the first step, the influence of pH, temperature, carbon source, nitrogen source and inducers on lipase activity was investigated by the One-Factor-At-A-Time (OFAT) method. In the second step, the three significant factors resulted from OFAT were optimized by the statistical approach (CCD). The optimum values of olive oil (0.5%), Tween 80 (0.6%) and FeSO4 (0.05%) was found to be responsible for a 3.2-fold increase in the lipase production identified by Central Composite Design.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available