Journal
BIOCONJUGATE CHEMISTRY
Volume 27, Issue 2, Pages 302-308Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.bioconjchem.5b00259
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Funding
- RAC, University of New Mexico
- Michael J. Fox Foundation for Parkinson's Research RIRA Program
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Fluorogenic sensors capable of spatiotemporally detecting Fe2+ in biological systems are highly valuable in the study of iron biology. Toward this end, a new off on Fe selective fluorescent probe has been developed by incorporating an Fe2+-induced N O cleavage of acylated hydroxylamine moiety into the naphthalimide fluorophore. The probe displays facile response (within 15 min) and good selectivity toward Fe2+ with >27-fold enhancement of fluorescence intensity and high sensitivity of as low as 0.5 mu M with a noticeable 3-fold fluorescence enhancement. These features of the probe have been transformed into in the convenient detection of endogenous, basal level of labile Fe2+ pools in living cells. Furthermore, we have demonstrated the capacity of the probe for the studies of important Fe2+ related biological functions. It can respond to the Zn(2+)induced Fe2+ flux, an important event observed in stroke, and facilely detect the elevated level of Fe' in the brain tissue of a rat undergoing ischemic stroke at the ischemic site.
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