Journal
ADVANCED MATERIALS
Volume 29, Issue 10, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/adma.201604245
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Funding
- BBSRC [BB/N012658/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [1270860, BB/N012658/1] Funding Source: researchfish
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In a new, versatile approach to functionalizing recombinant spider silk, L-azidohomoalanine is introduced residue-specifically in the minispidroin protein 4RepCT through expression in an E. coli methionine auxotroph. Both fluorophores and the antibiotic levofloxacin are attached to this bio-orthogonal amino acid using copper-catalyzed click chemistry, either before or after the silk fibers are self-assembled.
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