Journal
FRONTIERS IN ONCOLOGY
Volume 8, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fonc.2018.00447
Keywords
microRNA; FFPE (formalin-fixed paraffin-embedded); droplet digital PCR (ddPCR); cancer; EvaGreen chemistry
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Funding
- Italian Association for Cancer Research (AIRC) [IG 18464]
- Fondazione Pallotti (University of Bologna)
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Archival formalin-fixed paraffin-embedded (FFPE) tissues represent an extraordinary source of smalIRNAs, including microRNAs (miRNAs). Contrary to other RNA molecules, miRNAs are stable, nuclease-resistant and quantifiable even in low quality samples. The accurate assessment of miRNA levels in archival samples is of great interest for many pathological conditions, including cancer. In human tumors, microRNA expression is type-specific and can be used as diagnostic, prognostic or response-to-treatment biomarker. In this study, we provide a method for multiple miRNA quantification in 96-well plates, using EvaGreen-based droplet digital PCR technology and miRCURY LNA miRNA assays. This approach allows the absolute quantification of a customizable panel of miRNAs at the same time and under identical experimental conditions, to be used for diagnostic or prognostic applications.
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