Journal
JOURNAL OF CLINICAL MEDICINE
Volume 7, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/jcm7110449
Keywords
plant extracts; reprogramming; induced pluripotent stem cell; wound healing
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Funding
- National Research Foundation of Korea [2017R1A2B2012190]
- National Research Council of Science and Technology [CRC-15-02-KRIBB]
- Ministry of Food and Drug Safety [18172BIO182]
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Small molecules that improve reprogramming, stem cell properties, and regeneration can be widely applied in regenerative medicine. Natural plant extracts represent an abundant and valuable source of bioactive small molecules for drug discovery. Natural products themselves or direct derivatives of them have continued to provide small molecules that have entered clinical trials, such as anticancer and antimicrobial drugs. Here, we tested 3695 extracts from native plants to examine whether they can improve induced pluripotent stem cell (iPSC) generation using genetically homogeneous secondary mouse embryonic fibroblasts (MEFs) harboring doxycycline (dox)-inducible reprograming transgenes. Among the tested extracts, extracts from the fruit and stem of Camellia japonica (CJ) enhanced mouse and human iPSC generation and promoted efficient wound healing in an in vivo mouse wound model. CJ is one of the best-known species of the genus Camellia that belongs to the Theaceae family. Our findings identified the natural plant extracts from the fruit and stem of CJ as novel regulators capable of enhancing cellular reprogramming and wound healing, providing a useful supplement in the development of a more efficient and safer method to produce clinical-grade iPSCs and therapeutics.
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