Journal
ACS SENSORS
Volume 4, Issue 2, Pages 498-503Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.8b01573
Keywords
insulin; aptamers; sensors; G-quadruplex; electrochemistry
Funding
- National Institute Of Diabetes And Digestive And Kidney Diseases of the National Institutes of Health [UC4DK116283]
- University of Cincinnati McMicken Undergraduate STEM Research Award
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In this paper, we developed an electrochemical, aptamer-based (E-AB) for the real-time monitoring of insulin. The sensor utilizes a redox label-modified guanine-rich aptamer which folds into a G-quadruplex for specific recognition of insulin. To develop a reproducible E-AB sensor employing insulin aptamer probes for the detection of insulin, 10% sodium dodecyl sulfate (SDS) pretreatment is crucial as it disrupts interstrand G-quartets. After sensor pretreatment with 10% SDS, a more uniform sensor response is obtained. Upon introduction of the insulin target, binding-induced steric hindrance quantitatively reduces the efficiency of electron transfer of a distal-end redox label leading to the rapid signal change within similar to 60 s. Testing demonstrates that the E-AB insulin exhibits a limit of detection of 20 nM and can be used to discriminate against both glucagon and somatostatin in Krebs-Ringer bicarbonate buffer, typically used in perfusion experiments. These results demonstrate that this assay has potential for rapid, specific, and quantitative analysis of insulin.
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