Journal
ACS SENSORS
Volume 4, Issue 1, Pages 180-184Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.8b01202
Keywords
carbohydrates; rcSso7d; emulsions; Zika virus; Janus particles; sensors
Funding
- National Institutes of Health of General Medical Sciences [GM095843]
- MIT Tata Center for Technology and Design, Singapore's National Research Foundation via the Singapore-MIT Alliance for Research and Technology (AMR IRG)
- National Cancer Institute [P30CCA14051]
- MIT's Deshpande Center for Technological Innovation
- German Research Foundation (DFG) [ZE1121/1-1]
- National Science Foundation [1122374]
- Funai Overseas Scholarship
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A new class of Janus emulsion agglutination assay is reported for the detection of interfacial protein protein interactions. Janus emulsion droplets are functionalized with a thermally stable, antigen binding protein rcSso7d variant (rcSso7d-ZNS1) for the detection of Zika NS1 protein. The emulsion droplets containing fluorescent dyes in their hydrocarbon and fluorocarbon phases intensify the intrinsic optical signal with the emission intensity ratio, which can be detected by a simple optical fiber. This assay provides an opportunity for the in-field detection of Zika virus and other pathogens with a stable, inexpensive, and convenient device.
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