Journal
SCIENTIFIC DATA
Volume 5, Issue -, Pages -Publisher
NATURE PUBLISHING GROUP
DOI: 10.1038/sdata.2018.260
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Funding
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [P20GM121310] Funding Source: NIH RePORTER
- NIGMS NIH HHS [P20 GM121310] Funding Source: Medline
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Olfactory experience can alter the molecular and cellular composition of chemosensory neurons within the olfactory sensory epithelia of mice. We sought to investigate the scope of cellular and molecular changes within a mouse's olfactory system as a function of its exposure to complex and salient sets of odors: those emitted from members of the opposite sex. We housed mice either separated from members of the opposite sex (sex-separated) or together with members of the opposite sex (sex-combined) until six months of age, resulting in the generation of four cohorts of mice. From each mouse, the main olfactory epithelium (MOE), vomeronasal organ (VNO), and olfactory bulb (OB) were removed and RNA-extracted. A total of 36 RNA samples, representing three biological replicates per sex/condition/tissue combination, were analyzed for integrity and used to prepare RNA-seq libraries, which were subsequently analyzed via qPCR for the presence of tissue-or sex-specific markers. Libraries were paired-end sequenced to a depth of similar to 20 million fragments per replicate and the data were analyzed using the Tuxedo suite.
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