Journal
AMB EXPRESS
Volume 9, Issue -, Pages -Publisher
SPRINGEROPEN
DOI: 10.1186/s13568-018-0728-7
Keywords
Mandarin orange wastes; Mesophilic Clostridia; IBE fermentation; Consolidated bioprocessing
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Funding
- NEDO [25A2005]
- Sumitomo Foundation [114004]
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For a resolution of reducing carbon dioxide emission and increasing food production to respond to the growth of global population, production of biofuels from non-edible biomass is urgently required. Abundant orange wastes, such as peel and strained lees, are produced as by-product of orange juice, which is available non-edible biomass. However, d-limonene included in citrus fruits often inhibits yeast growth and makes the ethanol fermentation difficult. This study demonstrated that isopropanol-butanol-ethanol fermentation ability of Clostridium beijerinckii and cellulosic biomass degrading ability of C. cellulovorans were cultivated under several concentrations of limonene. As a result, C. cellulovorans was able to grow even in the medium containing 0.05% limonene (v/v) and degraded 85% of total sugar from mandarin peel and strained lees without any pretreatments. More interestingly, C. beijerinckii produced 0.046g butanol per 1g of dried strained lees in the culture supernatant together with C. cellulovorans.
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