Journal
STEM CELL REPORTS
Volume 12, Issue 2, Pages 213-229Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2018.12.011
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Funding
- European Research Council (ERC) [2012-StG-311736-PD-HUMMODEL]
- Spanish Ministry of Economy and Competitiveness (MINECO) [FIS2016-78507-C2-2-P, SAF2015-69706-R, BFU2016-80870-P]
- Instituto de Salud Carlos III (ISCIII/FEDER) (Red de Terapia Celular [TerCel]) [RD16/0011/0024]
- AGAUR [2014-SGR-878, 2017-SGR-899]
- CERCA Program/Generalitat de Catalunya
- PD-HUMMODEL ERC-Ideas PhD fellowship
- Spanish Ministry of Education (MEC) [FPU12/03332]
- MINECO [BES-2014-069603]
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Parkinson's disease (PD) is associated with the degeneration of ventral midbrain dopaminergic neurons (vmDAns) and the accumulation of toxic alpha-synuclein. A non-cell-autonomous contribution, in particular of astrocytes, during PD pathogenesis has been suggested by observational studies, but remains to be experimentally tested. Here, we generated induced pluripotent stem cell-derived astrocytes and neurons from familial mutant LRRK2 G2019S PD patients and healthy individuals. Upon co-culture on top of PD astrocytes, control vmDAns displayed morphological signs of neurodegeneration and abnormal, astrocyte-derived alpha-synuclein accumulation. Conversely, control astrocytes partially prevented the appearance of disease-related phenotypes in PD vmDAns. We additionally identified dysfunctional chaperone-mediated autophagy (CMA), impaired macroautophagy, and progressive alpha-synuclein accumulation in PD astrocytes. Finally, chemical enhancement of CMA protected PD astrocytes and vmDAns via the clearance of alpha-synuclein accumulation. Our findings unveil a crucial non-cell-autonomous contribution of astrocytes during PD pathogenesis, and open the path to exploring novel therapeutic strategies aimed at blocking the pathogenic cross talk between neurons and glial cells.
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