Journal
STEM CELL REPORTS
Volume 11, Issue 6, Pages 1551-1564Publisher
CELL PRESS
DOI: 10.1016/j.stemcr.2018.11.008
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Funding
- Stem Cell Network [DT3, DT4]
- Canadian Foundation for Innovation [33644]
- Michael Smith Foundation for Health Research (MSFHR) [5238 BIOM]
- CIHR-BC Transplantation Trainee Program
- BC Children's Hospital Research Institute
- University of British Columbia
- National Science and Engineering Research Council of Canada
- JDRF
- MSFHR
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Human embryonic stem cells (hESCs) are a potential unlimited source of insulin-producing beta cells for diabetes treatment. A greater understanding of how beta cells form during embryonic development will improve current hESC differentiation protocols. All pancreatic endocrine cells, including b cells, are derived from Neurog3-expressing endocrine progenitors. This study characterizes the single-cell transcriptomes of 6,905 mouse embryonic day (E) 15.5 and 6,626 E18.5 pancreatic cells isolated from Neurog3-Cre; Rosa26(mT/mG) embryos, allowing for enrichment of endocrine progenitors (yellow; tdTomato + EGFP) and endocrine cells (green; EGFP). Using a NEUROG3-2A-eGFP CyT49 hESC reporter line (N5-5), 4,462 hESC-derived GFP+ cells were sequenced. Differential expression analysis revealed enrichment of markers that are consistent with progenitor, endocrine, or previously undescribed cell-state populations. This study characterizes the single-cell transcriptomes of mouse and hESC-derived endocrine progenitors and serves as a resource (https://lynnlab.shinyapps.io/embryonic_pancreas) for improving the formation of functional beta-like cells from hESCs.
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