Journal
GENES
Volume 10, Issue 1, Pages -Publisher
MDPI
DOI: 10.3390/genes10010071
Keywords
Cabbage (Brassica oleracea var. capitata L.); expression profile; phylogenetic analysis; clubroot disease response; sugar transporter protein (STP)
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Funding
- China Postdoctoral Science Foundation [2017M621669]
- National Natural Science Foundation of China [31701773]
- National Key Research and Development Program of China [2016YFD0101702]
- Key Research and Development Program of Jiangsu Province [BE2017379]
- Jiangsu Agriculture Science and Technology Innovation Fund [CX(18)2006]
- National Modern Agriculture Industry Technology System [CARS-25]
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Sugar transporter protein (STP) genes are involved in multiple biological processes, such as plant responses to various stresses. However, systematic analysis and functional information of STP family genes in Brassica oleracea are very limited. A comprehensive analysis was carried out to identify BoSTP genes and dissect their phylogenetic relationships and to investigate the expression profiles in different organs and in response to the clubroot disease. A total of 22 BoSTP genes were identified in the B. oleracea genome and they were further classified into four clades based on the phylogenetic analysis. All the BoSTP proteins harbored the conserved sugar transporter (Sugar_tr, PF00083) domain, and the majority of them contained 12 transmembrane helices (TMHs). Rates of synonymous substitution in B. oleracea relative to Arabidopsis thaliana indicated that STP genes of B. oleracea diverged from those of A. thaliana approximately 16.3 million years ago. Expression profiles of the BoSTP genes in different organs derived from RNA-Seq data indicated that a large number of the BoSTP genes were expressed in specific organs. Additionally, the expression of BoSTP4b and BoSTP12 genes were induced in roots of the clubroot-susceptible cabbage (CS-JF1) at 28 days after inoculation with Plasmodiophora brassicae, compared with mock-inoculated plants. We speculated that the two BoSTPs might be involved in monosaccharide unloading and carbon partitioning associated with P. brassicae colonization in CS-JF1. Subcellular localization analysis indicated that the two BoSTP proteins were localized in the cell membrane. This study provides insights into the evolution and potential functions of BoSTPs.
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