Journal
FRONTIERS IN PHARMACOLOGY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fphar.2018.01579
Keywords
Ginkgo biloba; hydrolysis; ACE inhibitory activity; purification and identification; molecular docking
Categories
Funding
- Major Projects of Science and Technology in Anhui Province [17030701024, 17030701058, 17030701028]
- Key Research and Development Project of Anhui Province [1704g07020110, 1804b06020347]
Ask authors/readers for more resources
Alcalase, dispase, trypsin, and flavourzyme were used to hydrolyze the extracted Ginkgo biloba seeds protein isolate (GPI). The Ginkgo protein hydrolyzates (GPHs) with the maximum degree of hydrolysis (DH) and ACE inhibitory activity were selected, and ultra-filtered to obtain components with different molecular weights (MW) (<1 kDa, 1-3, 3-5, and 5-10 kDa). The components with MW of <1 kDa showed better ACE inhibition (IC50:0.2227 mg/mL). Purification and identification by Sephadex G-15 gel chromatography and LC-MS/MS conferred three new potential ACE inhibitory peptides [TNLDWY (non-competitive suppression mode), IC50:1.932 mM; RADFY (competitive inhibition modes), IC50:1.35 mM; RVFDGAV (competitive inhibition modes), IC50:1.006 mM]. Molecular docking depicting the inhibitory mechanism for ACE inhibitory peptides indicated that the peptides bound well to ACE and interacted with amino acid residues at the ACE active site.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available