Journal
BOTANICAL STUDIES
Volume 59, Issue -, Pages -Publisher
SPRINGER
DOI: 10.1186/s40529-018-0246-6
Keywords
Wintersweet; Squalene; Squalene synthase
Categories
Funding
- National Natural Science Foundation of China [31470693, 31100503]
- Natural Science Foundation of Zhejiang Province [LY18C160006]
- Central Public-interest Scientific Institution Basal Research Fund [1610212018004, 1610212016017]
- Science and Technology Innovation Project of the Chinese Academy of Agricultural Sciences [CAAS-ASTIP-2014-TRICAAS]
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BackgroundThree species of wintersweets: Chimonanthus salicifolius S. Y. Hu, Chimonanthus zhejiangensis M. C. Liu and Chimonanthus grammalus M. C. Liu are widely distributed in China. The three wintersweets belonging to the genus of Chimonanthus that can synthesize abundant terpenoids that are beneficial to human health. Their buds and leaves are traditional Chinese herb applied by the She' ethnic minority in southeast of China. Squalene is a multi-functional and ubiquitous triterpene in plants, which is biosynthesized by squalene synthase (SQS) using farnesyl diphosphate (FPP) as the substrate. The synthesis of squalene in wintersweet was not clearly. This work would provide us much help to further understand the terpene metabolism in wintersweet and its health function to people at phytochemistry and molecular levels.ResultsIn this study, we identified squalene component in the extractions of leaves of three wintersweets and isolated SQS genes from leaf transcriptomes. The three SQSs were highly conservative, so CzSQS from C. zhejiangensis was just determined the enzymatic activity. The in vitro expressed CzSQS that deleted two transmembrane domains could catalyze FPP to generate squalene with the presence of NADPH and Mg2+.ConclusionsThe squalene was one of wintersweet leaves phytochemicals. The squalene synthases of three wintersweet plants were highly conserved. The CzSQS was capable to catalyze two FPP molecules to squalene.
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