Journal
LIGHT-SCIENCE & APPLICATIONS
Volume 7, Issue -, Pages -Publisher
CHINESE ACAD SCIENCES, CHANGCHUN INST OPTICS FINE MECHANICS AND PHYSICS
DOI: 10.1038/s41377-018-0111-0
Keywords
-
Categories
Funding
- University of Dundee
- Scottish Universities Physics Alliance (PaLS initiative)
- European Regional Development Fund [CZ. 02.1.01/0.0/0.0/15 003/0000476]
- John Fell Fund
- BBSRC (TDRF)
- MRC (UK)
- BBSRC [BB/P02730X/1] Funding Source: UKRI
- MRC [G0701061] Funding Source: UKRI
Ask authors/readers for more resources
Achieving intravital optical imaging with diffraction-limited spatial resolution of deep-brain structures represents an important step toward the goal of understanding the mammalian central nervous system(1-4). Advances in wavefront-shaping methods and computational power have recently allowed for a novel approach to high-resolution imaging, utilizing deterministic light propagation through optically complex media and, of particular importance for this work, multimode optical fibers (MMFs)(5-7). We report a compact and highly optimized approach for minimally invasive in vivo brain imaging applications. The volume of tissue lesion was reduced by more than 100-fold, while preserving diffraction-limited imaging performance utilizing wavefront control of light propagation through a single 50-mu m-core MMF. Here, we demonstrated high-resolution fluorescence imaging of subcellular neuronal structures, dendrites and synaptic specializations, in deep-brain regions of living mice, as well as monitored stimulus-driven functional Ca2+ responses. These results represent a major breakthrough in the compromise between high-resolution imaging and tissue damage, heralding new possibilities for deep-brain imaging in vivo.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available