Journal
FRONTIERS IN MICROBIOLOGY
Volume 9, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fmicb.2018.02998
Keywords
laccase; thermostability; organic solvent tolerance; halotolerance; dye decolorization
Categories
Funding
- Innovation and Strengthening School Project from Guangdong Pharmaceutical University [2016SFKC_28, 2016KTSCX067]
- National Natural Science Foundation of China [31400680]
- School Research Fund from Anhui Medical University [2017xkj007]
- Science and Technology Plan Project of Guangdong Province [2014A020208134, 2017A010105011]
- Education Project of Guangdong Province [2013KJCX0107]
- Science and Technology Plan Project of Guangzhou [201802030009]
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Synthetic dyes are widely used in many industries, but they cause serious environmental problems due to their carcinogenic and mutagenic properties. In contrast to traditional physical and chemical treatments, biodegradation is generally considered an environmental-friendly, efficient, and inexpensive way to eliminate dye contaminants. Here, a novel laccase-like enzyme Lac1326 was cloned from a marine metagenomic library. It showed a maximum activity at 60 degrees C, and it retained more than 40% of its maximal activity at 10 degrees C and more than 50% at 20-70 degrees C. Interestingly, the laccase behaved stably below 50 degrees C, even in commonly used water-miscible organic solvents. The enzyme decolorized all tested dyes with high decolorization efficiency. This thermostable enzyme with high decolorization activity and excellent tolerance of organic solvents and salt has remarkable potential for bioremediation of dye wastewater. It is thus proposed as an industrial enzyme.
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