Journal
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
Volume 6, Issue 11, Pages 15720-15726Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.8b04235
Keywords
Nonspecific DNA; Sensor array; Cu-Au nanoparticles; Calorimetric; Protein discrimination
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Funding
- Scientific Research Project of Beijing Educational Committee [KM201710028009]
- Capacity Building for Sci-Tech Innovation-Fundamental Scientific Research Funds [025185305000/195]
- Youth Innovative Research Team of Capital Normal University
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Here, for the first time, we present a colorimetric sensor array toward sensitive protein discrimination based on a novel strategy of diverse DNA-protein interactions with the performance of iodide responsive Cu-Au nanoparticles (NPs). Different steric hindrance effects generated from differential interactions of DNA and proteins inhibit the accessibility of iodide to the NP surfaces, generating diverse colorimetric signals as fingerprints associated with each specific protein, which is subjected to linear discriminant analysis (LDA), a pluralistic statistic analysis technique for assignment of unknown samples to their suitable categories and data classification, to generate a clustering map. On the basis of the LDA analysis, eight proteins with a concentration of 20 nM in pure aqueous buffer and in real serum samples were successfully discriminated with 100% accuracy.
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