Journal
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
Volume 7, Issue 3, Pages 3339-3348Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssuschemeng.8b05489
Keywords
Metal-organic framework; Cellulose; Biocatalysis; beta-Glucosidase; Immobilized enzymes
Categories
Funding
- National Natural Science Foundation of China [21507047, 21676124]
- Programs of Senior Talent Foundation of Jiangsu University [14JDG053]
Ask authors/readers for more resources
Enhancing the stability and reusability of enzymes by encapsulating them within a powerful class of porous materials termed metal-organic frameworks (MOFs) has been demonstrated by recent studies. However, in all of these reports, the stability of MOFs under acidic conditions was a major issue for enzymes with catalytic activity at low pH. The objective of this study is to encapsulate the beta-glucosidase (beta-G) into the Cu-MOF due to its acidic stability, using a coprecipitation approach. Indeed, the as-prepared beta-G@Cu(PABA) biocomposite with high encapsulation efficiency exhibited enhanced resistance to acidic and thermal conditions as well as organic solvents. Importantly, the beta-G@Cu(PABA) biocomposite showed superior reusability, retaining 90% of activity even after 10 cycles. Using the hydrolysis of carboxymethylcellulose (CMC) as a mode, the mixture of beta-G@Cu(PABA) biocomposite and coimmobilized cellulase afforded 98% glucose yield, which was twofold enhancement when compared with coimmobilized cellulose alone. The beta-G@Cu(PABA) biocomposite and coimmobilized cellulase could been easily recovered and recycled to still retain 70% productivity in the eight cycles for hydrolyzing CMC. This discovery for the first time highlights the potential of Cu-MOF in preserving the enzymes under acidic conditions. The developed beta-G@Cu(PABA) biocomposite by coprecipitation approach affords a valuable platform for enzyme-based industrial applications.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available