Journal
MOLECULAR BRAIN
Volume 11, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s13041-018-0414-3
Keywords
Super-resolution fluorescence microscopy; Single-molecule localization microscopy; FRET; FRET-PAINT
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Funding
- Creative Research Initiative grant (Physical Genetics Laboratory) [2009-0081562]
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Recent development of FRET-PAINT microscopy significantly improved the imaging speed of DNA-PAINT, the previously reported super-resolution fluorescence microscopy with no photobleaching problem. Here we try to achieve the ultimate speed limit of FRET-PAINT by optimizing the camera speed, dissociation rate of DNA probes, and bleed-through of the donor signal to the acceptor channel, and further increase the imaging speed of FRET-PAINT by 8-fold. Super-resolution imaging of COS-7 microtubules shows that high-quality 40-nm resolution images can be obtained in just tens of seconds.
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