Journal
JOURNAL OF ANALYTICAL METHODS IN CHEMISTRY
Volume 2019, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2019/9371790
Keywords
-
Categories
Funding
- NSFC Projects of International Cooperation and Exchanges [2015DFA31810]
- Natural Science Foundation of Shanghai City, China [17411972400]
- Fund of innovation for doctor candidates of Second Military Medical University [20162109]
Ask authors/readers for more resources
Colorectal cancer is the most common critical disease both in the developed and developing countries. Capecitabine, which has served in clinical practice at least for 10 years, is a first-line antidigestive tract cancer drug for its better efficacy, patient compliance, and lower side effects. An ultra-high-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method has been developed and completely validated for simultaneous determination of capecitabine and its five metabolites in human plasma from colorectal cancer patients after administration of capecitabine tablet. One-step liquid-liquid extraction was successfully applied using ethyl acetate and isopropanol (19:1, V:V) for sample pretreatment. Chromatographic separation was achieved within 5min based on an Atlantis T3-C-18 column (3.0 mu m, 2.1x100mm) with gradient elution using mobile phases consisting of 0.0075% formic acid in water (pH 4) and in acetonitrile, and the flow rate was 0.3mL/min. Linear range was approximately 20.0-5000.0ng/mL for all analytes. Linear correlation coefficients were >0.99 for all regression curves. The intraday and interday accuracy and precision of the method were within +/- 15.0% and less than 15.0%, respectively. The mean recovery and matrix effect as well as stability of all the analytes ranged from 59.27% to 90.15% and from 74.84% to 114.48% as well as within +/- 15.0%. This simple, rapid, and sensitive method was successfully applied in 42 sparse clinical samples to verify its practicability.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available