Journal
G3-GENES GENOMES GENETICS
Volume 9, Issue 1, Pages 81-94Publisher
OXFORD UNIV PRESS INC
DOI: 10.1534/g3.118.200726
Keywords
Drosophila; mushroom body; transcriptome; RNA-seq
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Funding
- US National Institutes on Deafness and Other Communication Disorders [5R01DC013071-06]
- DART NeuroScience LLC
- Taiwan National Science Council [102-2917-I-564-004]
- US National Institute of Arthritis and Musculoskeletal and Skin Diseases
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The insect mushroom body (MB) is a conserved brain structure that plays key roles in a diverse array of behaviors. The Drosophila melanogaster MB is the primary invertebrate model of neural circuits related to memory formation and storage, and its development, morphology, wiring, and function has been extensively studied. MBs consist of intrinsic Kenyon Cells that are divided into three major neuron classes (, / and /) and 7 cell subtypes (d, m, /ap, /m, /p, /s and /c) based on their birth order, morphology, and connectivity. These subtypes play distinct roles in memory processing, however the underlying transcriptional differences are unknown. Here, we used RNA sequencing (RNA-seq) to profile the nuclear transcriptomes of each MB neuronal cell subtypes. We identified 350 MB class- or subtype-specific genes, including the widely used / class marker Fas2 and the / class marker trio. Immunostaining corroborates the RNA-seq measurements at the protein level for several cases. Importantly, our data provide a full accounting of the neurotransmitter receptors, transporters, neurotransmitter biosynthetic enzymes, neuropeptides, and neuropeptide receptors expressed within each of these cell types. This high-quality, cell type-level transcriptome catalog for the Drosophila MB provides a valuable resource for the fly neuroscience community.
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