Journal
CELL REPORTS
Volume 25, Issue 7, Pages 1772-+Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2018.10.059
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Funding
- National Medical Research Council (NMRC) Grant of Singapore Translational Research Investigator Award [NMRC/STaR/0019/2014]
- KAKEN [18K08364, 26221309, 26713035, 15KK0350]
- NMRC Clinician Scientist-Individual Research Grant (CS-IRG) New Investigator Grant [NMRC/CNIG/1164/2017]
- Grants-in-Aid for Scientific Research [15KK0350, 18K08364, 26713035, 26221309] Funding Source: KAKEN
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During acute myelosuppression or thrombocytopenia, bone marrow (BM) hematopoietic cells respond rapidly to replenish peripheral blood platelets. While the cytokine thrombopoietin (Thpo) both regulates platelet production and maintains HSC potential, whether Thpo controls megakaryocyte (Mk)-lineage differentiation of HSCs is unclear. Here, we show that Thpo rapidly upregulates mitochondrial activity in HSCs, an activity accompanied by differentiation to an Mk lineage. Moreover, in unperturbed hematopoiesis, HSCs with high mitochondrial activity exhibit Mk-lineage differentiation in vitro and myeloid lineage-biased reconstitution in vivo. Furthermore, Thpo skewed HSCs to express the tetraspanin CD9, a pattern correlated with mitochondrial activity. Mitochondrial-active HSCs are resistant to apoptosis and oxidative stress upon Thpo stimulation. Thpo-regulated mitochondrial activity associated with mitochondrial translocation of STAT3 phosphorylated at serine 727. Overall, we report an important role for Thpo in regulating rapid Mk-lineage commitment. Thpo-dependent changes in mitochondrial metabolism prime HSCs to undergo direct differentiation to an Mk lineage.
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