Journal
CELL REPORTS
Volume 25, Issue 1, Pages 38-+Publisher
CELL PRESS
DOI: 10.1016/j.celrep.2018.09.011
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Funding
- Ontario Institute for Cancer Research (OICR) [IA- 026]
- Canadian Institutes of Health Research (CIHR operating grant) [MOP-130294]
- CIHR Banting and Best doctoral student award [GSD-140321]
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The planarian epidermis provides an excellent model to explore adult stem cell (ASC) lineage development due to well-characterized and distinct spatiotemporal phases during lineage progression. Using flow cytometry-isolated cells enriched in epidermal progenitors, we performed transcriptional profiling and RNAi screening to uncover regulators of epidermal differentiation. We identified a MYB-type transcription factor (Smed-myb-1) required for the specification of the first temporal phase of post-mitotic maturation. Knockdown of myb-1 abolished the early progenitor phase of differentiation without ceasing production of subsequent epidermal progenitor states or homeostatic turnover and regeneration of the epidermis. Further examination revealed accelerated maturation of ASC descendants, with premature entry into subsequent progeny phases and, ultimately, the epidermis. These results demonstrate that a spatiotemporal shift in lineage progression occurs in the absence of the early progenitor state after myb-1 RNAi, and identify myb-1 as a critical regulator of the early temporal window in stepwise specification during planarian epidermal differentiation.
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