Journal
ADVANCED HEALTHCARE MATERIALS
Volume 8, Issue 5, Pages -Publisher
WILEY
DOI: 10.1002/adhm.201801226
Keywords
3D electrophysiology; glycine receptors; melt electrowriting
Funding
- German Research Foundation (DFG) as part of the Collaborative Research Centre [SFB TRR225, INST 93/940-1]
- German Research Foundation (DFG) as part of the State Major Instrumentation Program [INST 105022/58-1 FUGG]
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2D electrophysiology is often used to determine the electrical properties of neurons. In the brain however, neurons form extensive 3D networks. Thus, performing electrophysiology in a 3D environment provides a closer situation to the physiological condition and serves as a useful tool for various applications in the field of neuroscience. In this study, 3D electrophysiology is established within a fiber-reinforced matrix to enable fast readouts from transfected cells, which are often used as model systems for 2D electrophysiology. Using melt electrowriting (MEW) of scaffolds to reinforce Matrigel, 3D electrophysiology is performed on a glycine receptor-transfected Ltk-11 mouse fibroblast cell line. The glycine receptor is an inhibitory ion channel associated when mutated with impaired neuromotor behavior. The average thickness of the MEW scaffold is 141.4 +/- 5.7 mu m, using 9.7 +/- 0.2 mu m diameter fibers, and square pore spacings of 100, 200, and 400 mu m. For the first time, the electrophysiological characterization of glycine receptor-transfected cells is demonstrated with respect to agonist efficacy and potency in a 3D matrix. With the MEW scaffold reinforcement not interfering with the electrophysiological measurement, this approach can now be further adapted and developed for different kinds of neuronal cultures to study and understand pathological mechanisms under disease conditions.
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