Journal
ACS SYNTHETIC BIOLOGY
Volume 8, Issue 2, Pages 455-462Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssynbio.8b00427
Keywords
synthetic biology; protein expression; cell-free transcription and translation; PURE system
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Funding
- Ecole Polytechnique Federale de Lausanne
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We demonstrate a simple, robust, and low-cost method for producing the PURE cell-free transcription translation system. Our OnePot PURE system achieved a protein synthesis yield of 156 peg/mL at a cost of 0.09 USD/ktL, leading to a 14-fold improvement in cost normalized protein synthesis yield over existing PURE systems. The one pot method makes the PURE system easy to generate and allows it to be readily optimized and modified.
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