Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
Volume 1853, Issue 6, Pages 1416-1428Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamcr.2014.11.020
Keywords
Iron-sulfur cluster biosynthesis; Conformational equilibria; Protein-protein interactions; Nuclear magnetic resonance (NMR) spectroscopy; Small-angle X-ray scattering (SAM)
Categories
Funding
- NIH [U01 GM94622, P41RR02301, P41GM66326, S10RR02781, S10RR08438, S10RR023438, S10RR025062, S10RR029220]
- University of Wisconsin-Madison
- NSF [DMB-8415048, OIA-9977486, BIR-9214394]
- USDA
- Ruth L. Kirschstein National Research Service Award [F32 GM110939]
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Proteins containing iron-sulfur (Fe-S) clusters arose early in evolution and are essential to life. Organisms have evolved machinery consisting of specialized proteins that operate together to assemble Fe-S clusters efficiently so as to minimize cellular exposure to their toxic constituents: iron and sulfide ions. To date, the best studied system is the iron-sulfur cluster (isc) operon of Escherichia coli, and the eight ISC proteins it encodes. Our investigations over the past five years have identified two functional conformational states for the scaffold protein (IscU) and have shown that the other ISC proteins that interact with IscU prefer to bind one conformational state or the other. From analyses of the NMR spectroscopy-derived network of interactions of ISC proteins, small-angle X-ray scattering (SAXS) data, chemical crosslinking experiments, and functional assays, we have constructed working models for Fe-S cluster assembly and delivery. Future work is needed to validate and refine what has been learned about the E. coli system and to extend these findings to the homologous Fe-S cluster biosynthetic machinery of yeast and human mitochondria. This article is part of a Special Issue entitled: Fe/S proteins: Analysis, structure, function, biogenesis and diseases. (C) 2014 The Authors. Published by Elsevier B.V.
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