Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
Volume 1853, Issue 8, Pages 1850-1859Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamcr.2015.04.021
Keywords
Tom70; Mitochondria; Protein import; Presequence
Categories
Funding
- Deutsche Forschungsgemeinschaft [SFB860, FOR 1086/2, SCHW 823/2-1, SR7000DC]
- PhD program 'Molecular Biology' - International Max Planck Research School
- Gottingen Graduate School for Neurosciences and Molecular Biosciences (GGNB) (DFG) at the Georg August University Gottingen [GSC 226/1]
- Boehringer Ingelheim Fonds
- Max Planck Society
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The translocase of the outer mitochondrial membrane (TOM complex) is the general entry gate into mitochondria for almost all imported proteins. A variety of specific receptors allow the TOM complex to recognize targeting signals of various precursor proteins that are transported along different import pathways. Aside from the well-characterized presequence receptors Tom20 and Tom22 a third TOM receptor, Tom70, binds proteins of the carrier family containing multiple transmembrane segments. Here we demonstrate that Tom70 directly binds to presequence peptides using a dedicated groove. A single point mutation in the cavity of this pocket (M551R) reduces the presequence binding affinity of Tom70 ten-fold and selectively impairs import of the presequence-containing precursor Mdl1 but not the ADP/ATP carrier (MC). Hence Tom70 contributes to the presequence import pathway by recognition of the targeting signal of the Mdl1 precursor. (C) 2015 Elsevier B.V. All rights reserved.
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