Journal
BIOMEDICAL OPTICS EXPRESS
Volume 9, Issue 11, Pages 5703-5718Publisher
OPTICAL SOC AMER
DOI: 10.1364/BOE.9.005703
Keywords
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Funding
- National Research Foundation - Korean government (MSIP) [NRF-2018R1A2B6007635]
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2016R1D1A3B03931548]
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We demonstrated the apoptotic effect of bee venom (BV) on human MDA-MB231 breast cancer cells using Raman spectroscopy and principal component analysis (PCA). Biochemical changes in cancer cells were monitored following BV treatment; the results for different concentrations and treatment durations differed markedly. Significantly decreased Raman vibrations for DNA and proteins were observed for cells treated with 3.0 mu g/mL BV for 48 h compared with those of control cells. These results suggest denaturation and degradation of proteins and DNA fragmentation (all cell death-related processes). The Raman spectroscopy results agreed with those of atomic force microscopy and conventional biological tests such as viability, TUNEL. and western blot assays. Therefore. Raman spectroscopy, with PCA, provides a noninvasive, label-free tool for assessment of cellular changes on the anti-cancer effect of BV. (C) 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
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