Journal
ASIA-PACIFIC JOURNAL OF CHEMICAL ENGINEERING
Volume 14, Issue 1, Pages -Publisher
WILEY
DOI: 10.1002/apj.2278
Keywords
Alcaligenes faecalis JF339228; DNA interaction; immobilised spent tea activated carbon; phenol and cyanide; toxicity evaluation
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Funding
- Ministry of Human resource Development (MHRD), Government of India [SR/FST/ETI-405/2015]
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The current study is designed for the eradication of phenol and cyanide from a simulated binary mixture involving acetic acid modified spent tea activated carbon (STAC-C), isolated bacterial strain Alcaligenes faecalis JF339228, and immobilized A. faecalis JF339228 onto spent tea biochar by an amalgamated approach of sorption coupled with biodegradation. Characterisation of the biosorbent was ascertained by field emission scanning electron microscopy, thermogravimetric analysis, and Fourier transform infrared spectroscopy. The viability for eco-benign expulsion of the pernicious pollutants, and the resultant compounds after treatment was examined by fluorescence and UV-visible spectrophotometry with comprehensive elucidation of the interactions with DNA. Gas chromatography-mass spectrometry (GC-MS) anatomization was conducted for the evaluation of degraded derivatives post-treatment. The chemical and structural analysis of the degraded compounds and its impact upon disposal on the basis of degree of toxicity and reactivity were evaluated using TOXTREE software. Batch studies were implemented for the evaluation of process parameters like pH, reaction time, biosorbent or inoculum dosage, initial concentration, and temperature. Mono and binary component isotherm modelling was executed. Immobilised cells and free cells could accomplish better removal efficacy of 94.71% and 92.25% for cyanide, respectively, whereas adsorption using STAC-C was responsible for maximum removal of phenol up to 86.29%.
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