Journal
NATURE COMMUNICATIONS
Volume 9, Issue -, Pages -Publisher
NATURE RESEARCH
DOI: 10.1038/s41467-018-06768-z
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Funding
- French National Research Agency through the program Investments for the Future (France-BioImaging) [ANR-10-INBS-04]
- Commissariat aux Grands Investissements [ANR-10-INBS-09-03, ANR-10-INBS-09-02]
- ANR [ANR-11-BSV2-021-02, ANR-13-BSV4-0013, ANR-15-CE13-0003]
- FRM [DEQ20141231765, DEQ20130326464, DEQ20180339158]
- Fondation ARC [PJA 20161204865, PJA 20161204542]
- labex Signalife [ANR-11-LABX-0028-01]
- association Vaincre la Mucoviscidose [RF20140501158, RF20120600738, RF20150501288]
- Chan Zuckerberg Initiative (Silicon Valley Fundation) [2017-175159-5022]
- Ligue Nationale contre le Cancer
- Canceropole PACA
- Agence Nationale de la Recherche (ANR) [ANR-13-BSV4-0013, ANR-15-CE13-0003] Funding Source: Agence Nationale de la Recherche (ANR)
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Multiciliated cells (MCCs) harbor dozens to hundreds of motile cilia, which generate hydrodynamic forces important in animal physiology. In vertebrates, MCC differentiation involves massive centriole production by poorly characterized structures called deuterosomes. Here, single-cell RNA sequencing reveals that human deuterosome stage MCCs are characterized by the expression of many cell cycle-related genes. We further investigated the uncharacterized vertebrate-specific cell division cycle 20B (CDC20B) gene, which hosts microRNA-449abc. We show that CDC20B protein associates to deuterosomes and is required for centriole release and subsequent cilia production in mouse and Xenopus MCCs. CDC20B interacts with PLK1, a kinase known to coordinate centriole disengagement with the protease Separase in mitotic cells. Strikingly, over-expression of Separase rescues centriole disengagement and cilia production in CDC20B-deficient MCCs. This work reveals the shaping of deuterosome-mediated centriole production in vertebrate MCCs, by adaptation of canonical and recently evolved cell cycle-related molecules.
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