Journal
NATURE COMMUNICATIONS
Volume 10, Issue -, Pages -Publisher
NATURE PORTFOLIO
DOI: 10.1038/s41467-018-07845-z
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Funding
- 'France Genomique' consortium [ANR-10-INBS-0009]
- Labex Ecofect of the Universite de Lyon, within the program Investissements d'Avenir [ANR-11-LABX-0048, ANR-11-IDEX-0007]
- Fondation FINOVI
- Agence Nationale des Recherches sur le SIDA et les Hepatites Virales [ANRS-ECTZ3306]
- European Research Council under the European Union's Horizon 2020 research and innovation programs [ERC-StG-LS6-805500]
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Programmable nucleases have enabled rapid and accessible genome engineering in eukaryotic cells and living organisms. However, their delivery into target cells can be technically challenging when working with primary cells or in vivo. Here, we use engineered murine leukemia virus-like particles loaded with Cas9-sgRNA ribonucleoproteins (Nanoblades) to induce efficient genome-editing in cell lines and primary cells including human induced pluripotent stem cells, human hematopoietic stem cells and mouse bone-marrow cells. Transgene-free Nanoblades are also capable of in vivo genome-editing in mouse embryos and in the liver of injected mice. Nanoblades can be complexed with donor DNA for all-in-one homology-directed repair or programmed with modified Cas9 variants to mediate transcriptional up-regulation of target genes. Nanoblades preparation process is simple, relatively inexpensive and can be easily implemented in any laboratory equipped for cellular biology.
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