Journal
REVIEWS IN AQUACULTURE
Volume 12, Issue 1, Pages 365-381Publisher
WILEY
DOI: 10.1111/raq.12322
Keywords
CRISPR; Cas9; CRISPRi; genetic manipulation; microalgae; synthetic biology
Categories
Funding
- A4HW project by the Spanish Ministry of Economy, Industry and Competitiveness under the Collaboration Challenges programme [RTC-2016-4860-2]
- European Union
- ENDESA Generaci~on, S.A.
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Knowledge at the molecular level of microalgae has obtained great scientific interest in recent years, because of their potential for biofuel production, as well as other highly valued molecules. Recent advances in the area of the genetic manipulation of microalgae open an entirely new field of possible applications for these organisms as biorefineries. This review represents a compendium that gathers the main milestones that have marked the area of genetic engineering in microalgae during the last decade, from classical techniques for the transformation and expression of transgenes, to the most avant-garde techniques currently used for gene silencing and DNA edition, as well as a discussion about the future perspectives in this field.
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