Journal
BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH
Volume 1853, Issue 1, Pages 111-125Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamcr.2014.10.005
Keywords
Peroxisome; Ustilago maydis; Fatty acid beta-oxidation; Mitochondria; Filamentous fungus; Organelle biogenesis
Categories
Funding
- Portuguese Foundation for Science and Technology (FCT)
- FEDER/COMPETE [PTDC/SAU-OSM/103647/2008, PTDC/BIA-BCM/118605/2010]
- BBSRC [BB/K006231/1, SFRH/BPD/74428/2010, SFRH/BD/73532/2010, SFRH/BPD/90084/2012]
- CRUP/Treaty of Windsor (ACCOES INTEGRADAS) [B-33/09]
- Biotechnology and Biological Sciences Research Council [BB/K006231/1] Funding Source: researchfish
- BBSRC [BB/K006231/1] Funding Source: UKRI
- Fundação para a Ciência e a Tecnologia [SFRH/BPD/90084/2012, SFRH/BD/73532/2010, SFRH/BPD/74428/2010, PTDC/SAU-OSM/103647/2008, PTDC/BIA-BCM/118605/2010] Funding Source: FCT
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Peroxisomes are ubiquitous organelles which participate in a variety of essential biochemical pathways. An intimate interrelationship between peroxisomes and mitochondria is emerging in mammals, where both organelles cooperate in fatty acid beta-oxidation and cellular lipid homeostasis. As mitochondrial fatty acid beta-oxidation is lacking in yeast and plants, suitable genetically accessible model systems to study this interrelationship are scarce. Here, we propose the filamentous fungus Ustilago maydis as a suitable model for those studies. We combined molecular cell biology, bioinformatics and phylogenetic analyses and provide the first comprehensive inventory of U. maydis peroxisomal proteins and pathways. Studies with a peroxisome-deficient Delta pex3 mutant revealed the existence of parallel and complex, cooperative beta-oxidation pathways in peroxisomes and mitochondria, mimicking the situation in mammals. Furthermore, we provide evidence that acyl-CoA dehydrogenases (ACADs) are bona fide peroxisomal proteins in fungi and mammals and together with acyl-CoA oxidases (ACOX) belong to the basic enzymatic repertoire of peroxisomes. A genome comparison with baker's yeast and human gained new insights into the basic peroxisomal protein inventory shared by humans and fungi and revealed novel peroxisomal proteins and functions in U. maydis. The importance of our findings for the evolution and function of the complex interrelationship between peroxisomes and mitochondria in fatty acid beta-oxidation is discussed. (C) 2014 Elsevier B.V. All rights reserved.
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