Journal
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
Volume 34, Issue 11, Pages -Publisher
SPRINGER
DOI: 10.1007/s11274-018-2549-x
Keywords
Antifungal; Candida albicans; Alpha-glucosidase; Magnoflorine; Susceptibility microdilution assay
Categories
Funding
- Basic Science Research Program from the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2015R1D1A1A01061225]
- IPET [116094-03-1-SB010]
Ask authors/readers for more resources
Candida albicans is a major invasive pathogen, and the development of strains resistant to conventional antifungal agents has been reported in recent years. We evaluated the antifungal activity of 44 compounds against Candida strains. Magnoflorine showed the highest growth inhibitory activity of the tested Candida strains, with a minimum inhibitory concentration (MIC) of 50g/mL based on microdilution antifungal susceptibility testing. Disk diffusion assay confirmed the antifungal activity of magnoflorine and revealed that this activity was stable over 3days compared to those of berberine and cinnamaldehyde. Cytotoxicity testing showed that magnoflorine could potentially be used in a clinical setting because it didn't have any toxicity to HaCaT cells even in 200g/mL of treatment. Magnoflorine at 50g/mL inhibited 55.91 +/- 7.17% of alpha-glucosidase activity which is required for normal cell wall composition and virulence of Candida albicans. Magnoflorine also reduced the formation of C. albicans' biofilm. Combined treatment with magnoflorine and miconazole decreased the amount of miconazole required to kill various Candida albicans. Therefore, magnoflorine is a good candidate lead compound for novel antifungal agents.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available