Journal
VIROLOGY JOURNAL
Volume 15, Issue -, Pages -Publisher
BMC
DOI: 10.1186/s12985-018-1105-5
Keywords
Zika virus; Seroepidemiology; Virus neutralization test
Categories
Funding
- European Union's Horizon 2020 Research and Innovation Programme under ZIKAlliance [734548]
- European Virus Archive EVAg
- European Union [653316]
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Here we propose a strategy allowing implementing efficient and practicable large-scale seroepidemiological studies for Zika Virus (ZIKV). It combines screening by a commercial NS1 protein-based Zika IgG ELISA, and confirmation by a cytopathic effect-based virus neutralization test (CPE-based VNT). In post-epidemic samples from Martinique Island blood donors (a population with a dengue seroprevalence above 90%), this strategy allowed reaching specificity and sensitivity values over 98%. The CPE-based VNT consists of recording CPE directly under the optical microscope, which is easy to identify with ZIKV strain H/PF/2013 at day 5 pi. Overall, considered that CPE-based VNT is cost effective and widely automatable, the NS1 protein-based Zika IgG ELISA+CPE-based VNT combination strategy represents a convenient tool to expedite ZIKV seroprevalence studies.
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