Journal
TROPICAL JOURNAL OF PHARMACEUTICAL RESEARCH
Volume 17, Issue 9, Pages 1833-1838Publisher
PHARMACOTHERAPY GROUP
DOI: 10.4314/tjpr.v17i9.22
Keywords
Respiratory tract infection; Interleukin; Taraxacum mongolicum; Immunity; Neonatal rats
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Purpose: To study the antimicrobial activity of the Taraxacum mongolicum extract against respiratory infection-causing bacterial strains in vitro and in neonatal rats. Methods: The in vitro antibacterial activity was assessed by micro-dilution method. Antioxidant activity was determined by ferric reducing antioxidant power (FRAP), nitro blue tetrazolium (NBT) and 2, 2diphenyl-1-picrylhydrazyl (DPPH) assays. In vivo antimicrobial activity was evaluated in neonatal rat model. Interleukin (IL)-2 (IL-2) and gamma interferon (IFN-gamma) were estimated using enzyme-linked immunosorbent assay (ELISA). Results: The hydro-methanol extract of T. mongolicum contained high levels of phenolics and flavonoids, and exhibited strong antimicrobial activity against respiratory infection-causing bacterial species with MICs of 25 -100 mu g/ml, and MBCs of 55 -215 mu g/ml. The highest and lowest antimicrobial activities were observed against Streptococcus pneumonia and Haemophilus influenza, respectively. The extract at doses of 25 and 50 mg/kg exerted protective effects against Streptococcus pneumonia-infected neonatal rats by boosting their Th1 immunity. It enhanced the production of interleukin (IL)-2, concomitant with decreased production of interferon (IFN)-gamma in neonatal rats. The extract contained isoetin, hesperidin, naringenin, kaempferol, sinapinic and gallic acid. Conclusion: These results suggest that the hydro-methanolic extract of Taraxacum mongolicum and its constituents can be potentially developed for use in the management of respiratory bacterial infections.
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