A method of detecting two tumor markers (p-hydroxybenzoic acid and p-cresol) in human urine using a porous magnetic < beta > -cyclodextrine polymer as solid phase extractant, an alternative for early gastric cancer diagnosis

Analyzing of tumor markers has become an important means for cancer diagnosis and prevention. In this study, a novel solid phase extraction based on porous magnetic cyclodextrin polymer (MA-CD) was developed and used for detection of trace small molecule gastric tumor markers in urine samples. The adsorption properties of the magnetic cyclodextrin polymer were tested. Through experiments of the solid phase extraction (SPE) at the different condition, the optimal condition was selected to test the two tumor markers by High-performance liquid chromatography -Diode array detector (HPLC-DAD). The analytical performance of the method showed good accuracy (88.82%-104.34%) and precision (< 3.55%), appropriated detection limits (1.016 and 5.714 mu g L-1) and linear ranges (0.6-24.0 mu g L-1) with convenient determination coefficients (> 0.9994). The results demonstrated that the developed approach is efficient, low-cost for gastric tumor markers detection.