Journal
SMALL
Volume 15, Issue 4, Pages -Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/smll.201804216
Keywords
3D bioprinting; cell encapsulation; gelatin methacryloyl (GelMA); microdroplet fabrication; tissue engineering
Categories
Funding
- National Nature Science Foundation of China [51622510, U1609207, 51805474]
- Science Fund for Creative Research Groups of the National Natural Science Foundation of China [51521064]
- Nature Science Foundation of Zhejiang Province, China [LR17E050001]
- Fundamental Research Funds for the Central Universities
- Key Research and Development Program of Zhejiang Province [21 2017C01063]
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Low-concentration gelatin methacryloyl (GelMA) has excellent biocompatibility to cell-laden structures. However, it is still a big challenge to stably fabricate organoids (even microdroplets) using this material due to its extremely low viscosity. Here, a promising electro-assisted bioprinting method is developed, which can print low-concentration pure GelMA microdroplets with low cost, low cell damage, and high efficiency. With the help of electrostatic attraction, uniform GelMA microdroplets measuring about 100 mu m are rapidly printed. Due to the application of lower external forces to separate the droplets, cell damage during printing is negligible, which often happens in piezoelectric or thermal inkjet bioprinting. Different printing states and effects of printing parameters (voltages, gas pressure, nozzle size, etc.) on microdroplet diameter are also investigated. The fundamental properties of low-concentration GelMA microspheres are subsequently studied. The results show that the printed microspheres with 5% w/v GelMA can provide a suitable microenvironment for laden bone marrow stem cells. Finally, it is demonstrated that the printed microdroplets can be used in building microspheroidal organoids, in drug controlled release, and in 3D bioprinting as biobricks. This method shows great potential use in cell therapy, drug delivery, and organoid building.
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