Journal
SENSORS
Volume 18, Issue 11, Pages -Publisher
MDPI
DOI: 10.3390/s18114032
Keywords
hepatocellular carcinoma; alpha-fetoprotein; biosensor; graphene; field-effect transistor
Funding
- MSIT (Ministry of Science and ICT), Korea, under the Global IT Talent support program [IITP-2017-0-01811]
- Gumi Core Components and Materials Technology Development Program of the Gumi Regional Government, 2016
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The detection of alpha-fetoprotein (AFP) in plasma is important in the diagnosis of hepatocellular carcinoma (HCC) in humans. We developed a biosensor to detect AFP in HCC patient plasma and in a phosphate buffer saline (PBS) solution using a graphene field-effect transistor (G-FET). The G-FET was functionalized with 1-pyrenebutyric acid N-hydroxysuccinimide ester (PBASE) for immobilization of an anti-AFP antibody. AFP was detected by assessing the shift in the voltage of the Dirac point (Delta V-Dirac) after binding of AFP to the anti-AFP-immobilized G-FET channel surface. This anti-AFP-immobilized G-FET biosensor was able to detect AFP at a concentration of 0.1 ng mL(-1) in PBS, and the detection sensitivity was 16.91 mV. In HCC patient plasma, the biosensor was able to detect AFP at a concentration of 12.9 ng mL(-1), with a detection sensitivity of 5.68 mV. The sensitivity (Delta V-Dirac) depended on the concentration of AFP in either PBS or HCC patient plasma. These data suggest that G-FET biosensors could have practical applications in diagnostics.
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