Journal
RESEARCH IN VETERINARY SCIENCE
Volume 121, Issue -, Pages 130-133Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.rvsc.2018.10.015
Keywords
Serum amyloid a; Haptoglobin; Endometritis; Lipopolysaccharide
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Funding
- Basal Research Budget Incremental Fund of Chinese Academy of Agricultural Sciences [2014ZL012]
- Central Public-interest Scientific Institution Basal Research Fund [1610322014001]
- Science and technology Innovation Project [CAAS-ASTIP-2014-LIHPS-03]
- National Key Research and Development Program of China [2017YFD0502200]
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This study investigated the correlation between endometrial local expressions of serum amyloid A (SAA) and haptoglobin (Hp) and severity of endometritis in 20 Chinese Holstein dairy cows at 40-60 days postpertum, aged 3-6, and 2-5 parities. Endometrial biopsies were collected and categorized into normal, mild endometritis, and severe endometritis based on clinical signs and histopathology. The protein and mRNA expression of SAA and Hp in endometrial tissue were measured using western blot and real-time PCR, respectively. Furthermore, bovine endometrial epithelial cells were isolated and cultured in vitro. The protein and mRNA expression of SAA and Hp were also determined in the cells treated with increasing concentrations of lipopolysaccharide (LPS; 0, 1, 5, 10 mu g/ml). The results showed that both of protein and mRNA of SAA was increased in endometritis or in LPS-stimulated cells, and the increases were positively correlated with severity of endometritis in vivo or LPS stimulation strength in vitro. Meanwhile, protein expression of Hp were also increased in endometritis or in LPS-stimulated cells, but the increases were negatively correlative with severity of inflammation as well as mRNA expression of Hp in vivo or in vitro. Therefore, our results suggest that endometrial local expression of SAA is more significant than Hp as a potential biomarker to assess severity of endometritis in cows.
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