Journal
PREPARATIVE BIOCHEMISTRY & BIOTECHNOLOGY
Volume 48, Issue 10, Pages 887-897Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/10826068.2018.1514516
Keywords
Encapsulated lipase; enzymatic kinetic resolution; magnetic nanoparticles; silane; sporopollenin; stability
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Funding
- Scientific Research Foundation of Selcuk University, Konya, Turkey (BAP)
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The aim of this study was to prepare the encapsulation of Candida rugosa lipase (CRL) with magnetic sporopollenin. The sporopollenin was covalent immobilized onto magnetic nanoparticles (Fe3O4), grafted amino (APTES), or epoxy groups (EPPTMS). CRL was sol-gel encapsulated in the presence of magnetic sporopollenin/Fe3O4 nanoparticles. The influence of activation agents ([3-(2,3-epoxypropoxy) propyl] trimethoxysilane (EPPTMS), (3-Aminopropyl)triethoxysilane (APTES) and pH and thermal stabilities of the biocatalyst were assessed. Experimental data showed the improved catalytic activity at different pH and temperature values. At 60 degrees C, free lipase lost its initial activity within 80 min of time, although the encapsulated lipases retained their initial activities of about 65% by APTES and 60% by EPPTMS after 120 min of heat treatment at 60 degrees C. The catalytic properties of the encapsulated lipases were utilized to hydrolysis of racemic aromatic carboxylic acid methyl esters (Naproxen and 2-phenoxypropionic acid). The results show that the sporopollenin-based encapsulated lipase (Fe-A-Spo-E) has greater enantioselectivity and conversion in comparison with the encapsulated lipase without supports (lipase-enc).
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