Journal
PLANT SCIENCE
Volume 278, Issue -, Pages 96-106Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.plantsci.2018.10.003
Keywords
Antioxidants; Lsi1; PAL2-2; Rice; Silicon; UV-B radiation
Categories
Funding
- National Natural Science Foundation of China [31300336]
- Provincial Natural Science Foundation of Fujian, China [2015J01079]
- Fujian-Taiwan Joint Innovative Centre for Germplasm Resources and Cultivation of Crop (China) [2015-75]
- Open Project Program of the Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, College of Crop Science, Fujian Agriculture and Forestry University [GBMUC-2018-001]
- Foundation for the Science & Technology Innovation of Fujian Agriculture and Forestry University [CXZX2017310, CXZX2017145, CXZX2017309]
- Foundation of Institute of Modern Seed Industrial Engineering
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Silicon (Si) enhances the resistance of rice to biotic and abiotic stress. In rice, the accumulation of Si is controlled by the low silicon rice 1 (Lsi1) gene; overexpression of Lsi1 (Lsi1-OX) increases Si uptake and accumulation, while the reverse is observed in Lsi1-RNA interference (Lsi1-RNAi) transgenic rice. When the two transgenic rice lines and wild-type (WT) rice were exposed to ultraviolet (UV)-B radiation, the Lsi1-OX or Lsi1-RNAi rice showed differential microRNA (miRNA) expression, compared to WT rice. These miRNAs were predicted to target genes involved in light signal transduction and cell detoxification. The greatest capacities of ascorbate peroxidase, superoxide dismutase, peroxidase, and phenylalanine ammonia lyase (PAL) and highest contents of phenolics, flavonoids, and proline were found in Lsi1-OX rice, followed by WT rice and Lsi1-RNAi transgenic rice. A further comparison of the transcript levels of individual PAL genes revealed that the expression of PAL2-2 (Os02g0626400) was positively regulated by Lsi1. Our results demonstrate that Lsi1 overexpression or interference causes changes in both miRNA expression and antioxidant capacity in rice, and therefore modulates rice tolerance to UV -B radiation. Furthermore, we demonstrated that PAL2-2 was positively regulated by Lsi1 during this process.
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