Journal
PLANT CELL AND ENVIRONMENT
Volume 42, Issue 4, Pages 1352-1367Publisher
WILEY
DOI: 10.1111/pce.13479
Keywords
airborne (Z)-3-hexenol; glucosyltransferase; overexpression; site-directed mutagenesis; tea plant
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Funding
- National Natural Science Foundation of China [31870678]
- Innovation Projects for the Returned Overseas Chinese Scholars of Anhui Province
- Key Projects of State Key Laboratory of Tea Plant Biology and Utilization [2016ZR022]
- Start-up Foundation for Advanced Talents of Anhui Agricultural University [yj2016-16]
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Plants emit a variety of volatiles in response to herbivore attack, and (Z)-3-hexenol and its glycosides have been shown to function as defence compounds. Although the ability to incorporate and convert (Z)-3-hexenol to glycosides is widely conserved in plants, the enzymes responsible for the glycosylation of (Z)-3-hexenol remained unknown until today. In this study, uridine-diphosphate-dependent glycosyltransferase (UGT) candidate genes were selected by correlation analysis and their response to airborne (Z)-3-hexenol, which has been shown to be taken up by the tea plant. The allelic proteins UGT85A53-1 and UGT85A53-2 showed the highest activity towards (Z)-3-hexenol and are distinct from UGT85A53-3, which displayed a similar catalytic efficiency for (Z)-3-hexenol and nerol. A single amino acid exchange E59D enhanced the activity towards (Z)-3-hexenol, whereas a L445M mutation reduced the catalytic activity towards all substrates tested. Transient overexpression of CsUGT85A53-1 in tobacco significantly increased the level of (Z)-3-hexenyl glucoside. The functional characterization of CsUGT85A53 as a (Z)-3-hexenol UGT not only provides the foundation for the biotechnological production of (Z)-3-hexenyl glucoside but also delivers insights for the development of novel insect pest control strategies in tea plant and might be generally applicable to other plants.
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