Journal
PEDIATRIC SURGERY INTERNATIONAL
Volume 35, Issue 1, Pages 3-7Publisher
SPRINGER
DOI: 10.1007/s00383-018-4369-3
Keywords
Neonatal intestinal injury; Intestinal organoids; Necrotizing enterocolitis; Stress factors
Categories
Funding
- Canadian Institutes of Health Research (CIHR) Foundation Grant [353857]
- HSC Restracomp Fellowship
Ask authors/readers for more resources
BackgroundAdult intestinal organoids have been used to study ex vivo intestinal injury in adulthood. However, the neonatal intestinal epithelium has many unique features that are different from adult mature intestine. Establishing a neonatal ex vivo organoid model is essential to study the epithelial physiology in early postnatal development and to investigate derangements associated with disease processes during the neonatal period like necrotizing enterocolitis (NEC).MethodsFresh and frozen terminal ileum was harvested from mice pups on postnatal day 9. Crypts were isolated and organoids were cultured. Organoids were exposed to hypoxia and lipopolysaccharide (LPS) for 48h to induce epithelial injury. Inflammatory cytokines and tight junction proteins were evaluated.ResultsRobust intestinal organoids can be formed from both fresh and frozen intestinal tissue of neonatal mice pups. Hypoxia and LPS administration induced intestinal inflammation and disrupted tight junctions in these neonatal intestinal organoids.ConclusionsWe have established a novel method to grow organoids from neonatal intestine. We demonstrated that these organoids respond to the injury occurring during neonatal intestinal diseases such as NEC by increasing the organoid inflammation and by disrupting the organoid barrier function. Organoids provide an ex vivo platform to study intestinal physiology and pathology during the neonatal period.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available