Journal
OZONE-SCIENCE & ENGINEERING
Volume 41, Issue 3, Pages 242-249Publisher
TAYLOR & FRANCIS INC
DOI: 10.1080/01919512.2018.1524285
Keywords
Ozone; Streptococcus mutans; Scanning Electron Microscopy; Atomic Force Microscopy; microbial viability; dental applications
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Ozonated water has been demonstrated to induce significant results in terms of the elimination of microorganisms. The present study assessed the damage to Streptococcus mutans after exposure to ozonated water; the ozone generator was adjusted to provide an outlet concentration of 60 mg/L, the samples were submitted to different ozonation times 1, 2, 4, 6, and 10 mi. Scanning electron microscopy and atomic force images were obtained to identify damage to the bacteria, followed by reactive oxygen species (ROS) evaluation and microbial viability. The results showed a significant reduction in viability and the images evidenced the generation of gaps on the microbial wall and surface layer alterations. Ozone can induce significant damage to S. mutans, thus suggesting that the use of ozonated water to prevent carious lesion formation is extremely promising.
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