Journal
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
Volume 2018, Issue -, Pages -Publisher
HINDAWI LTD
DOI: 10.1155/2018/9396089
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Funding
- National Natural Science Foundation of China [81770279, 31500649]
- major project of the Natural Science Research of Jiangsu Higher Education Institutions of China [18KJA310005]
- project of the Six Talent Peaks Project in Jiangsu Province [2018-WSN-062]
- China Postdoctoral Science Foundation [2017M610342]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [1701050A]
- research and innovation project of the Graduate Students in Jiangsu Province [KYCX18_2401, KYLX16_0979]
- Nantong University [YKC16055]
- Natural Science Foundation of Nantong City [MS12015015, MS12015101]
- Nantong University Cooperative Innovation Program of Small Molecular Compound RD [NTU2016-1]
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Hydrogen sulfide (H2S) is a gasotransmitter with a variety of cardiovascular protective effects. Sirtuin 3 (SIRT3) is closely related to mitochondrial function and oxidative stress. We found that NaHS increased SIRT3 expression in the preventive effect on isoproterenol- (ISO-) induced myocardial hypertrophy. We further investigated whether exogenous H2S supplement improved ISO-induced myocardial hypertrophy in a SIRT3-dependent manner. 10-week-old male 129S1/SvImJ (WT) mice and SIRT3 knockout (KO) mice were intraperitoneally injected with NaHS (50 mu mol/kg/d) for two weeks and then intraperitoneally injected with ISO (60 mg/kg/d) for another two weeks. In WT mice, NaHS significantly reduced the cardiac index of ISO-induced mice, decreased the cross-sectional area of cardiomyocytes, and inhibited the expressions of atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) mRNA. The activity of total antioxidant capacity (T-AOC) and superoxide dismutase (SOD) in the myocardium was increased, but the level of malondialdehyde (MDA) was decreased. The fluorescence intensity of dihydroethidium staining for superoxide anion was attenuated. Optic atrophy 1 (OPA1) expression was upregulated, while dynamin-related protein 1 (DRP1) expression was downregulated. ERK, but not P38 and JNK, phosphorylation was downregulated. However, all above protective effects were unavailable in ISO-induced SIRT3 KO mice. Our present study suggested that exogenous H2S supplement inhibited ISO-induced cardiac hypertrophy depending on SIRT3, which might be associated with antioxidant stress.
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