Journal
ORAL ONCOLOGY
Volume 86, Issue -, Pages 251-257Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.oraloncology.2018.09.030
Keywords
Exosome; Extracellular vesicle; Epithelial-mesenchymal transition; Epidermal growth factor receptor; Oral cancer; Head and neck cancer
Categories
Funding
- JSPS KAKENHI [JP17K11642, 17K11643, JP16K11722, JP17K11669]
- Grants-in-Aid for Scientific Research [17K11643] Funding Source: KAKEN
Ask authors/readers for more resources
Overexpression and increased signaling from the epidermal growth factor receptor (EGFR) often changes oral squamous cell carcinoma (OSCC) and thus EGFR is frequently targeted molecularly by the therapeutic antibody cetuximab. We assessed the roles of OSCC-derived extracellular vesicles (EVs), including exosomes in the trafficking of cetuximab and in epithelial-mesenchymal transition (EMT) of epithelial cells. OSCC cells abundantly expressed EGFR, which was secreted from cells with OSCC-EVs upon EGF stimulations. The OSCC-EGFR-EVs were then able to enter into and transform epithelial cells leading to increased mesenchymal traits with increased vimentin and spindle-like shapes. EGF priming of OSCC cells further increased this EMT-initiating effect of the OSCC-EVs. The internalization and pro-EMT effects of the OSCC-EVs were largely blocked by cetuximab. Thus, OSCC-derived EVs transform normal epithelial cells into a mesenchymal phenotype and anti-EGFR therapeutic antibody cetuximab inhibits such a carcinogenic effect of the OSCC-EVs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available